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            手性拆分篩選和純化試劑盒
            手性化合物結晶分離技術是一種從外消旋混合物中獲得光學純手性化合物的常規方法,但該技術的難點在于拆分試劑和溶劑的篩選與優化組合,為了找到優組合,往往要進行一系列耗時、枯燥而耗力的工作。而Maybridge 手性拆分篩選和純化試劑盒正是針對這一技術難點而開發的產品,它能夠幫助科學家們快速找到佳的拆分條件,高收率獲得高純度的手性化合物。

            產品優勢

            高性能—384種拆分試劑和溶劑組合,有助于找到佳拆分條件

            快速-篩選實驗可在一天內完成

            高效-少僅需消耗0.4mmol 外消旋混合物即可完成組合優化篩選

            即用-所有備選拆分試劑和溶劑均按順序預置于96孔板中,即取即用

            便捷-專為快遞篩選設計,陽性結果可肉眼觀測或光學檢測,并可輕松純化回收手性產物

            試劑盒工作原理

            外消旋混合物與另一手性化合物(常被稱為拆分試劑)作用可生成非對映異構體鹽混合物,當不同手性非對映異構體鹽的物理性質(如溶解度、吸附力等)差異較大時,就可以通過結晶法或色譜法對其進行分離、再生、純化,從而得到目標手性化合物。下面以伯胺為例進行說明。

            外消旋的伯胺混合物用光學純有機酸羧酸(也可以使用磺酸類化合物)處理生成非對映異構體的銨鹽。

            非對映異構體:

            調整溫度或其他外界條件后,其中一種非對映異構體鹽首先結晶,從溶液體系中析出,而另一種仍在溶劑中溶解。接下來可以通過過濾或重結晶的方法獲得目標手性化合物的非對映異構體,一般還需多次重結晶進一步純化目標手性化合物。

            結晶分離:

            最后,銨鹽被堿性水溶液中和后,得到目標手性化合物。

            再生純化:

            TOP
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